CONDENSED ADIPOSE TISSUES MEMBRANE AS POTENTIAL IMPLANTS FOR SOFT TISSUE REGENERATION
Authors:
Asaf Zigron1,2, Idan Redenski1,2, Daniel Oren1, Fares Kablan1, Samer Srouji1,2
Affiliation:
1 Galilee College of Dental Sciences, Oral and Maxillofacial Surgery Department, Galilee Medical Center, Nahariya, Israel
2 The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel
Doi: 10.54936/haoms242p58
ABSTRACT:
Background: Tissue engineering, aimed at producing fully functional tissue replacements, has provided promising alternatives for regenerating soft and hard tissue defects. Adipose tissues, which are abundant in the body, are readily accessible through liposuction or from the buccal or abdominal fat pads. These tissues are an excellent source of mesenchymal stromal cells (MSCs) and growth factors. Yet, extensive clinical use of these tissue sources as biological tissue grafts has yet to be reported. We have recently developed a novel technique to prepare adipose-derived membranes with tissue regeneration potential, able to withstand mechanical forces, and undergo tissue integration after implantation.
Methods: Adipose tissues were harvested from 350-400 gr rats and from healthy human subjects undergoing abdominoplasty and processed using a prototype apparatus for adipose tissue condensation. Condensed adipose tissue membranes from rats were used for a soft tissue implantation model. condensed adipose tissues from humans were tested for their mechanical properties, cellular sprouting and presence of MSCs. Condensed tissues were compared to uncondensed controls
Results: In vivo implantation of rat membranes indicated rapid perfusion and integration of condensed membranes opposed to uncondensed controls. Condensed membranes from human specimens exhibited superior mechanical characteristics and cellular sprouting, later characterized as stromal cells able to undergo differentiation toward adipose and osteogenic lineages. Preliminary randomized clinical trials of a human Buccal fat pad have demonstrated significant superiority of soft tissue healing.
Discussion: Preliminary results indicated that a rat in vivo model can be used to assess the contribution of adipose tissue condensation to defect bridging and remolding. Moreover, results from human specimens indicate the possibility of clinical translation.
KEY WORDS:
Authors:
Asaf Zigron1,2, Idan Redenski1,2, Daniel Oren1, Fares Kablan1, Samer Srouji1,2
Affiliation:
1 Galilee College of Dental Sciences, Oral and Maxillofacial Surgery Department, Galilee Medical Center, Nahariya, Israel
2 The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel
Doi: 10.54936/haoms242p58
ABSTRACT:
Background: Tissue engineering, aimed at producing fully functional tissue replacements, has provided promising alternatives for regenerating soft and hard tissue defects. Adipose tissues, which are abundant in the body, are readily accessible through liposuction or from the buccal or abdominal fat pads. These tissues are an excellent source of mesenchymal stromal cells (MSCs) and growth factors. Yet, extensive clinical use of these tissue sources as biological tissue grafts has yet to be reported. We have recently developed a novel technique to prepare adipose-derived membranes with tissue regeneration potential, able to withstand mechanical forces, and undergo tissue integration after implantation.
Methods: Adipose tissues were harvested from 350-400 gr rats and from healthy human subjects undergoing abdominoplasty and processed using a prototype apparatus for adipose tissue condensation. Condensed adipose tissue membranes from rats were used for a soft tissue implantation model. condensed adipose tissues from humans were tested for their mechanical properties, cellular sprouting and presence of MSCs. Condensed tissues were compared to uncondensed controls
Results: In vivo implantation of rat membranes indicated rapid perfusion and integration of condensed membranes opposed to uncondensed controls. Condensed membranes from human specimens exhibited superior mechanical characteristics and cellular sprouting, later characterized as stromal cells able to undergo differentiation toward adipose and osteogenic lineages. Preliminary randomized clinical trials of a human Buccal fat pad have demonstrated significant superiority of soft tissue healing.
Discussion: Preliminary results indicated that a rat in vivo model can be used to assess the contribution of adipose tissue condensation to defect bridging and remolding. Moreover, results from human specimens indicate the possibility of clinical translation.
KEY WORDS: