Squamous cell carcinoma of the tongue and microRNAs expression
Konstantinos PARASKEVOPOULOS, Panagiota TOUPLIKIOTI, Konstantinos VAHTSEVANOS, George KOLOUTSOS, Alexandros LAMBROPOULOS, Konstantinos ANTONIADES, Konstantinos PAPAZISIS
Department of Oral and Maxillofacial Surgery, School of Dentistry, Aristotle University of Thessaloniki, Greece (Head: Professor K. Antoniades)
Hellenic Archives of Oral & Maxillofacial Surgery (2019) 2, 49-56
SUMMARY: MicroRNAs are involved in regulating diverse cellular biological processes leading to malignancies, including tongue squamous cell carcinoma (TSCC). There are only few published data on the miRNAs that are ex- pressed in the squamous cell carcinoma of the tongue. The purpose of this study is to identify the differential miRNA expression between squamous cell carcinoma of the tongue and the adjacent normal tissues.
Material and methods: We have collected twelve samples from the tumor and the healthy adjacent tissue of the tongue. We used miRNA real-time PCR array system to identify miRNA expression profiles of squamous cell car- cinoma of the tongue.
Results and conclusions: We identified some deregulated miRNAs which may contribute to different prognosis. MiR-196a, miR-142, miR-96 and miR-21 were the ones with the highest upregulation, whilst the expression of miR-133, miR-206 and miR-1 was significantly suppressed. The target genes control pathways that regulate cell survival, proliferation, differentiation, migration, inva- sion, apoptosis and metabolism. It is therefore important to further explore their roles in the carcinogenesis and treatment of TSCC.
KEY WORDS: Tongue squamous cell carcinoma; human genome; microRNAs; regulation; target genes; pathways.
REFERENCES
Brandi N. Davis-Dusenbery, Akiko Hata: MicroRNA in Cancer: The Involvement of Aberrant MicroRNA Biogenesis Regulatory Path- ways. Genes & Cancer 1: 1100, 2010
Chan JA, Krichevsky AM, Kosik KS: MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 65(14):6029-33, 2005
Chen C, Zhang Y, Zhang L, Weakley SM, Yao Q: MicroRNA-196: crit- ical roles and clinical applications in development and cancer. J Cell Mol Med 15(1):14-23, 2011
Cheng Z, Liu F, Wang G, Li Y, Zhang H, Li F: MiR-133 is a key negative regulator of CDC42-PAK pathway in gastric cancer. Cell Signal 26(12):2667-2673, 2014
Fire A, Xu S, Montgomery M, Kostas SA, Driver SE, Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669):806–11, 1998
Franceschi D, Gupta R, Spiro RH, Shah JP: Improved survival in the treatment of squamous carcinoma of the oral tongue. Am J Surg 166:360–365, 1993
Garzon R, Calin GA, Croce CM: MicroRNAs in Cancer. Annu Rev Med 60:167-79, 2009
Guo Y, Liu H, Zhang H, Shang C, Song Y: MiR-96 regulates FOXO1- mediated cell apoptosis in bladder cancer. Oncol Lett 4(3):561- 565, 2012
Haflidadóttir BS, Larne O, Martin M, Persson M, Edsjö A, Bjartell A et al: Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1. PLoS One 8(8):e72400, 2013
Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ: Cancer statistics.
CA Cancer J Clin 57:43–66, 2007
Jinsong Li, Hongzhang Huang, Lijuan Sun, Yang M, Pan C, Chen W et al: MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor. Clin Cancer Res 15:3998- 4008, 2009
Luthra R, Singh RR, Luthra MG, Li YX, Hannah C, Romans AM et al: MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers. Oncogene 27(52):6667-78, 2008
Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST, Patel T: Mi- croRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology 133(2):647-58, 2007
Ni YH, Huang XF, Wang ZY, Han W, Deng RZ, Mou YB et al: Upreg- ulation of a potential prognostic biomarker, miR-155, enhances cell proliferation in patients with oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol 117(2):227-33, 2014
Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics, 2002. CA Cancer J Clin 55:74–108, 2005
Polanska H, Raudenska M, Gumulec J, Sztalmachova M, Adam V, Kizek R et al: Clinical significance of head and neck squamous cell cancer biomarkers. Oral Oncol 50(3):168-77, 2014
Ress AL, Stiegelbauer V, Winter E, Schwarzenbacher D, Kiesslich T, Lax S et al: MiR-96-5p influences cellular growth and is associated with poor survival in colorectal cancer patients. Mol Carcinog 54(11):1442-50, 2015
Santhi WS, Prathibha R, Charles S, Anurup KG, Reshmi G, Ramachan- dran S et al: Oncogenic microRNAs as biomarkers of oral tumori- genesis and minimal residual disease. Oral Oncol 49(6):567-75, 2013
Schetter AJ, Leung SY, Sohn JJ, Zanetti KA, Bowman ED, Yanaihara N et al: MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA 299(4):425-36, 2008
Si H, Sun X, Chen Y, Cao Y, Chen S, Wang Het al: Circulating mi- croRNA-92a and microRNA-21 as novel minimally invasive bio- markers for primary breast cancer. J Cancer Res Clin Oncol 139(2):223-9, 2013
Si ML, Zhu S, Wu H, Lu Z, Wu F, Mo YY: MiR-21-mediated tumor growth. Oncogene 26(19):2799-803, 2007
Singh A, Happel C, Manna SK, Acquaah-Mensah G, Carrerero J, Kumar S et al: Transcription factor NRF2 regulates miR-1 and miR-206 to drive tumorigenesis. J Clin Invest 123(7):2921-34, 2013
Su YH, Zhou Z, Yang KP, Wang XG, Zhu Y, Fa XE: MIR-142-5p and miR- 9 may be involved in squamous lung cancer by regulating cell cycle related genes. Eur Rev Med Pharmacol Sci 17(23):3213-20, 2013
Tao J, Wu D, Xu B, Qian W, Li P, Lu Q et al: MicroRNA-133 inhibits cell proliferation, migration and invasion in prostate cancer cells by targeting the epidermal growth factor receptor. Oncol Rep 27(6):1967-75, 2012
Yan LX, Huang XF, Shao Q, Huang MY, Deng L, Wu QL et al: Mi- croRNA miR-21 overexpression in human breast cancer is asso- ciated with advanced clinical stage, lymph node metastasis and patient poor prognosis. RNA 14(11):2348-60, 2008
Yu H, Lu Y, Li Z, Wang Q: MicroRNA-133: expression, function and therapeutic potential in muscle diseases and cancer. Curr Drug Targets 15(9):817-28, 2014
Zhang J, Kong X, Li J, Luo Q, Li X, Shen L et al: MiR-96 promotes tumor proliferation and invasion by targeting RECK in breast can- cer. Oncol Rep 31(3):1357-63, 2014
Zhang JG, Wang JJ, Zhao F, Liu Q, Jiang K, Yang GH: MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC). Clin Chim Acta 411(11-12):846-52, 2010
Zhang X., Z. Yan, J. Zhang, Gong L, Li W, Cui J et al: Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Annals of Oncology 22: 2257–2266, 2011
Zhang Y, Gan B, Liu D, Paik JH: FoxO family members in cancer. Can- cer Biol Ther 12(4):253-9, 2011
Zhou Y, Wu D, Tao J, Qu P, Zhou Z, Hou J: MicroRNA-133 inhibits cell proliferation, migration and invasion by targeting epidermal growth factor receptor and its downstream effector proteins in bladder cancer. Scand J Urol 47(5):423-32, 2013
Department of Oral and Maxillofacial Surgery, School of Dentistry, Aristotle University of Thessaloniki, Greece (Head: Professor K. Antoniades)
Hellenic Archives of Oral & Maxillofacial Surgery (2019) 2, 49-56
SUMMARY: MicroRNAs are involved in regulating diverse cellular biological processes leading to malignancies, including tongue squamous cell carcinoma (TSCC). There are only few published data on the miRNAs that are ex- pressed in the squamous cell carcinoma of the tongue. The purpose of this study is to identify the differential miRNA expression between squamous cell carcinoma of the tongue and the adjacent normal tissues.
Material and methods: We have collected twelve samples from the tumor and the healthy adjacent tissue of the tongue. We used miRNA real-time PCR array system to identify miRNA expression profiles of squamous cell car- cinoma of the tongue.
Results and conclusions: We identified some deregulated miRNAs which may contribute to different prognosis. MiR-196a, miR-142, miR-96 and miR-21 were the ones with the highest upregulation, whilst the expression of miR-133, miR-206 and miR-1 was significantly suppressed. The target genes control pathways that regulate cell survival, proliferation, differentiation, migration, inva- sion, apoptosis and metabolism. It is therefore important to further explore their roles in the carcinogenesis and treatment of TSCC.
KEY WORDS: Tongue squamous cell carcinoma; human genome; microRNAs; regulation; target genes; pathways.
REFERENCES
Brandi N. Davis-Dusenbery, Akiko Hata: MicroRNA in Cancer: The Involvement of Aberrant MicroRNA Biogenesis Regulatory Path- ways. Genes & Cancer 1: 1100, 2010
Chan JA, Krichevsky AM, Kosik KS: MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 65(14):6029-33, 2005
Chen C, Zhang Y, Zhang L, Weakley SM, Yao Q: MicroRNA-196: crit- ical roles and clinical applications in development and cancer. J Cell Mol Med 15(1):14-23, 2011
Cheng Z, Liu F, Wang G, Li Y, Zhang H, Li F: MiR-133 is a key negative regulator of CDC42-PAK pathway in gastric cancer. Cell Signal 26(12):2667-2673, 2014
Fire A, Xu S, Montgomery M, Kostas SA, Driver SE, Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391(6669):806–11, 1998
Franceschi D, Gupta R, Spiro RH, Shah JP: Improved survival in the treatment of squamous carcinoma of the oral tongue. Am J Surg 166:360–365, 1993
Garzon R, Calin GA, Croce CM: MicroRNAs in Cancer. Annu Rev Med 60:167-79, 2009
Guo Y, Liu H, Zhang H, Shang C, Song Y: MiR-96 regulates FOXO1- mediated cell apoptosis in bladder cancer. Oncol Lett 4(3):561- 565, 2012
Haflidadóttir BS, Larne O, Martin M, Persson M, Edsjö A, Bjartell A et al: Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1. PLoS One 8(8):e72400, 2013
Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ: Cancer statistics.
CA Cancer J Clin 57:43–66, 2007
Jinsong Li, Hongzhang Huang, Lijuan Sun, Yang M, Pan C, Chen W et al: MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor. Clin Cancer Res 15:3998- 4008, 2009
Luthra R, Singh RR, Luthra MG, Li YX, Hannah C, Romans AM et al: MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers. Oncogene 27(52):6667-78, 2008
Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST, Patel T: Mi- croRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology 133(2):647-58, 2007
Ni YH, Huang XF, Wang ZY, Han W, Deng RZ, Mou YB et al: Upreg- ulation of a potential prognostic biomarker, miR-155, enhances cell proliferation in patients with oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol 117(2):227-33, 2014
Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics, 2002. CA Cancer J Clin 55:74–108, 2005
Polanska H, Raudenska M, Gumulec J, Sztalmachova M, Adam V, Kizek R et al: Clinical significance of head and neck squamous cell cancer biomarkers. Oral Oncol 50(3):168-77, 2014
Ress AL, Stiegelbauer V, Winter E, Schwarzenbacher D, Kiesslich T, Lax S et al: MiR-96-5p influences cellular growth and is associated with poor survival in colorectal cancer patients. Mol Carcinog 54(11):1442-50, 2015
Santhi WS, Prathibha R, Charles S, Anurup KG, Reshmi G, Ramachan- dran S et al: Oncogenic microRNAs as biomarkers of oral tumori- genesis and minimal residual disease. Oral Oncol 49(6):567-75, 2013
Schetter AJ, Leung SY, Sohn JJ, Zanetti KA, Bowman ED, Yanaihara N et al: MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA 299(4):425-36, 2008
Si H, Sun X, Chen Y, Cao Y, Chen S, Wang Het al: Circulating mi- croRNA-92a and microRNA-21 as novel minimally invasive bio- markers for primary breast cancer. J Cancer Res Clin Oncol 139(2):223-9, 2013
Si ML, Zhu S, Wu H, Lu Z, Wu F, Mo YY: MiR-21-mediated tumor growth. Oncogene 26(19):2799-803, 2007
Singh A, Happel C, Manna SK, Acquaah-Mensah G, Carrerero J, Kumar S et al: Transcription factor NRF2 regulates miR-1 and miR-206 to drive tumorigenesis. J Clin Invest 123(7):2921-34, 2013
Su YH, Zhou Z, Yang KP, Wang XG, Zhu Y, Fa XE: MIR-142-5p and miR- 9 may be involved in squamous lung cancer by regulating cell cycle related genes. Eur Rev Med Pharmacol Sci 17(23):3213-20, 2013
Tao J, Wu D, Xu B, Qian W, Li P, Lu Q et al: MicroRNA-133 inhibits cell proliferation, migration and invasion in prostate cancer cells by targeting the epidermal growth factor receptor. Oncol Rep 27(6):1967-75, 2012
Yan LX, Huang XF, Shao Q, Huang MY, Deng L, Wu QL et al: Mi- croRNA miR-21 overexpression in human breast cancer is asso- ciated with advanced clinical stage, lymph node metastasis and patient poor prognosis. RNA 14(11):2348-60, 2008
Yu H, Lu Y, Li Z, Wang Q: MicroRNA-133: expression, function and therapeutic potential in muscle diseases and cancer. Curr Drug Targets 15(9):817-28, 2014
Zhang J, Kong X, Li J, Luo Q, Li X, Shen L et al: MiR-96 promotes tumor proliferation and invasion by targeting RECK in breast can- cer. Oncol Rep 31(3):1357-63, 2014
Zhang JG, Wang JJ, Zhao F, Liu Q, Jiang K, Yang GH: MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC). Clin Chim Acta 411(11-12):846-52, 2010
Zhang X., Z. Yan, J. Zhang, Gong L, Li W, Cui J et al: Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Annals of Oncology 22: 2257–2266, 2011
Zhang Y, Gan B, Liu D, Paik JH: FoxO family members in cancer. Can- cer Biol Ther 12(4):253-9, 2011
Zhou Y, Wu D, Tao J, Qu P, Zhou Z, Hou J: MicroRNA-133 inhibits cell proliferation, migration and invasion by targeting epidermal growth factor receptor and its downstream effector proteins in bladder cancer. Scand J Urol 47(5):423-32, 2013
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