MAXILLARY DISTRACTION OSTEOGENESIS
Authors:
Asst. Prof. Maria Papadaki
Affiliation:
European University Cyprus, Nicosia, Cyprus
Doi: 10.54936/haoms242p27
ABSTRACT:
Objectives: Distraction osteogenesis (DO) is a commonly used technique for skeletal expansion of the mandible and frequently reported
for midface expansion at the LeFort I and III levels. The purpose of this presentation is to describe new data on the biology of maxillary DO.
Materials and methods: 9 Minipigs underwent bilateral maxillary DO under general anesthesia. A Le Fort I osteotomy was performed and a distractor was fixed across the bone cut. A protocol of 0-day latency, 1 mm/day rate for 12 days and 24 days of fixation was followed. Maxillary specimens were harvested at end-DO (n=3), mid- fixation (n=3) and end fixation (n=3). Regenerate was stained with Hematoxylin/Eosin (H & E) and examined with light and fluorescence microscopy. In total, 216 fluorescence photographs were obtained from each study group. Results. Animals tolerated the procedure well. At end-DO group, more cells were noticed close to the natural bone and in the centre of the regenerate. Fibrous tissue decreased sequentially from the end-DO to the end-fixation groups. By the end of the distraction period there was active proliferation of pro-osteblasts and formation of bone trabeculae in continuation with the edges of the natural bone. Islands of chondrocyte-like cells appeared in one specimen each at mid- and end-fixation. PCNA index and vessel density were higher at the end-DO group compared to mid- fixation and end-fixation groups. Bone formation at the end of fixation period was complete based on clinical, radiographic and histological examination inDO can be all specimens. Fibrous tissue demonstrated strong fluorescence in H&E stained slides.
Conclusion: Despite differences in thickness of bone and morphology, the pattern of healing was similar to that described for the mandible. As in mandibular DO, intramembranous bone formation was the predominant mechanism of healing performed successfully in the maxilla with zero latency.
KEY WORDS:
Authors:
Asst. Prof. Maria Papadaki
Affiliation:
European University Cyprus, Nicosia, Cyprus
Doi: 10.54936/haoms242p27
ABSTRACT:
Objectives: Distraction osteogenesis (DO) is a commonly used technique for skeletal expansion of the mandible and frequently reported
for midface expansion at the LeFort I and III levels. The purpose of this presentation is to describe new data on the biology of maxillary DO.
Materials and methods: 9 Minipigs underwent bilateral maxillary DO under general anesthesia. A Le Fort I osteotomy was performed and a distractor was fixed across the bone cut. A protocol of 0-day latency, 1 mm/day rate for 12 days and 24 days of fixation was followed. Maxillary specimens were harvested at end-DO (n=3), mid- fixation (n=3) and end fixation (n=3). Regenerate was stained with Hematoxylin/Eosin (H & E) and examined with light and fluorescence microscopy. In total, 216 fluorescence photographs were obtained from each study group. Results. Animals tolerated the procedure well. At end-DO group, more cells were noticed close to the natural bone and in the centre of the regenerate. Fibrous tissue decreased sequentially from the end-DO to the end-fixation groups. By the end of the distraction period there was active proliferation of pro-osteblasts and formation of bone trabeculae in continuation with the edges of the natural bone. Islands of chondrocyte-like cells appeared in one specimen each at mid- and end-fixation. PCNA index and vessel density were higher at the end-DO group compared to mid- fixation and end-fixation groups. Bone formation at the end of fixation period was complete based on clinical, radiographic and histological examination inDO can be all specimens. Fibrous tissue demonstrated strong fluorescence in H&E stained slides.
Conclusion: Despite differences in thickness of bone and morphology, the pattern of healing was similar to that described for the mandible. As in mandibular DO, intramembranous bone formation was the predominant mechanism of healing performed successfully in the maxilla with zero latency.
KEY WORDS: